Cellulose Acetate Paper Electrophoresis

The electrophoresis on cellulose acetate membrane is most widely used because of its simplicity and is without the use of any sophisticated instrument other than electrophoresis apparatus and the cellulose acetate strip.

Cellulose acetate paper electrophoresis.

Cellulose acetate electrophoresis is more or less very similar to the paper electrophoresis. It is a modified version of paper electrophore sis developed by kohn in 1958. Widely used in analysis of clinical and biological protein sample albumin and globulins. First the cellulose acetate filter paper moistens with the buffer solution.

In this type of electrophoresis bacteriological acetate mem brane filters are taken in place of regular chro matography paper. Alternative to paper electrophoresis. Electrophoresis on cellulose acetate paper agarose and polyacrylamide has been used as a means of separating the lipoprotein classes and indeed the separation pattern on paper electrophoresis was the basis of the fredrickson classification of lipid disorders. Electrophoresis is typically performed at ph 8 6 using cellulose acetate as the support medium.

Cellulose acetate electrophoresis 23. Cellulose acetate is used as a film base in photography as a component in some coatings and as a frame material for eyeglasses. On cathode side of midline current 0 4 ma cm width of paper. Cellulose acetate is the acetate ester of cellulose it was first prepared in 1865.

It involves the following steps. 12x2 5 cm sample applied from micropipette or capillary along a line about 1 cm. Here we describe a modified version of cellulose acetate membrane electrophoresis for hemoglobin separation from blood sample. Cellulose acetate electrophoresis is an important technique in clinical diagnostics.

This new range of cellulose acetate products offers a complete system solution for research and clinical cellulose acetate electrophoresis procedures. Cellulose acetate electrophoresis it contains 2 3 acetyl groups per glucose unit and its adsorption capacity is less than that of paper. The cellulose acetate system is the ideal tank for both standard and wet cellulose acetate electrophoresis. It gives sharper bands.

It is also used as a synthetic fiber in the manufacture of cigarette filters and playing cards in photographic film cellulose acetate replaced nitrate film in the 1950s being far. Provides a good background or staining glycoprotein application. After this immerse both the ends of cellulose acetate filter paper in a buffer solution. The results were analyzed according to manufacturer s guidelines.